HP Data Explorer 4 Series Uživatelský manuál stáhnout pdf (Strana 352)

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Chapter 8 Viewing Voyager PSD Data

8-14 Applied Biosystems

NOTE: If the calibration reference file is stored on a

network drive, an error message may display when you

select the calibration file when performing a calibration.

If an error message is displayed, copy the file to a local

drive on your computer using Windows NT Explorer.

5. Enter Reference Matching Criteria:

• Minimum Intensity—Peaks must be above this

intensity to be considered a match. Select the unit

for Minimum Intensity, % Relative Intensity or

% Relative Area.

• Mass Tolerance—Peaks must be within this

tolerance of the theoretical mass to be considered

a match. Select the unit, m/z or ppm.

6. Select the Peak Weighting Factor. If the calibration

includes more than two points, you can apply the following

weighting factors to fit points to the curve:

• None—All peaks weighted equally

• Inverse Peak Width—Narrower peaks are

weighted more than broader peaks

• Height—More intense peaks are weighted more

than less intense peaks

7. Match observed peaks in the spectrum with reference

masses in the calibration reference file using one of the

following procedures:

•“Matching peaks automatically” described below

•“Selecting peaks manually” on page 8-16

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