HP Data Explorer 4 Series manuály

Hp Data Explorer 4 Series Uživatelský manuál (447 strany)

Značka: Hp | Kategorie: Software | Velikost: 3.67 MB |

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Data Explorer

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Software

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Data Explorer

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Software User’s Guide v

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Software User’s Guide vii

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Chapter 9 Troubleshooting

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(Visual Basic Macros)

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Table of Contents

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How to Use This Guide

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Notes, Cautions

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Warnings, and

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Send us your

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1 Data Explorer

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1.1 Overview

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Starting and

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1-4 Applied Biosystems

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1.2 File Formats and Types

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1-6 Applied Biosystems

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File Formats and Types

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Software User’s Guide 1-7

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Additional files

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Software User’s Guide 1-9

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1-10 Applied Biosystems

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1.3 Parts of the

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Data Explorer Window

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Software User’s Guide 1-13

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Context-sensitive

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Output window

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1.4 Customizing the

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1-18 Applied Biosystems

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Software User’s Guide 1-19

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Opening

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Applying a .SET

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1.4.3 Customizing Toolbars

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Undocking

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1.5 Setting Graphic Options

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Accessing

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Setting Graphic Options

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Software User’s Guide 1-25

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Setting line

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Setting data

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Setting traces in

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Line or Vertical

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Bar mode

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Setting graphic

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Software User’s Guide 1-29

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1.6 Managing Files

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Managing Files

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Software User’s Guide 1-31

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Viewing file

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Searching file

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1.6.2 Converting Data from

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1.6.3 Converting to and

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Exporting ASCII Data

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Importing a Trace

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Exporting .BIC

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.MSM, and .CAL

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Software User’s Guide 1-37

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Saving .LBS

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Copy displayed

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1-40 Applied Biosystems

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Software User’s Guide 1-41

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1-42 Applied Biosystems

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2 Using Chromatogram

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2.1.1 Opening Data Files

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2-4 Applied Biosystems

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Software User’s Guide 2-5

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Software User’s Guide 2-7

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Using Activate

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Software User’s Guide 2-9

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2.1.6 Closing Data Files

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Adjusting the Display Range

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Software User’s Guide 2-11

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2-12 Applied Biosystems

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2.3 Organizing Windows

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2.4 Manipulating Traces

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2.4.2 Duplicating a Trace

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2.4.4 Adding Traces from

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Setting the

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Replace mode

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2-18 Applied Biosystems

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Manipulating Traces

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Software User’s Guide 2-19

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2-20 Applied Biosystems

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2.4.5 Removing Traces

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(Processing History)

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History options

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2.4.8 Overlaying Traces

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Overlaying traces

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2-26 Applied Biosystems

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Software User’s Guide 2-27

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2.4.9 Annotating Traces

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Annotating the

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2.4.10 Viewing Trace Labels

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Spectrum trace

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2-32 Applied Biosystems

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2.4.11 Printing Traces

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2-34 Applied Biosystems

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Dedicating a

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2-36 Applied Biosystems

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2.5.2 Copying Traces from

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2.6 Saving, Opening, and

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Deleting .DAT Results

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2.7 Exporting, Opening, and

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Files (Mariner Data Only)

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.SPC Results Files

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Deleting results

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2-42 Applied Biosystems

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3 Peak Detection

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3.1 Overview

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3.1.2 The Resolution-Based

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Peak Detection Routine

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3-4 Applied Biosystems

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Voyager data

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Overlapping peak

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PSD peak

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3.2 Peak Detection

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Peak Detection

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Software User’s Guide 3-7

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3-8 Applied Biosystems

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Software User’s Guide 3-9

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3-10 Applied Biosystems

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Software User’s Guide 3-11

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3-12 Applied Biosystems

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Setting Basic

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Settings

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(spectrum data)

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3-14 Applied Biosystems

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Software User’s Guide 3-15

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Setting Peak

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Processing

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(spectrum data

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Setting Advanced

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Resetting Basic

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3.2.4 Peak Detection

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Parameter Descriptions

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Continued

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Software User’s Guide 3-21

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Software User’s Guide 3-25

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Software User’s Guide 3-27

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Software User’s Guide 3-31

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3.2.5 Charge State

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Determination and Examples

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Max Charge State

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3-34 Applied Biosystems

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Max Isotope #

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Effect of Minimum

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Intensity

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Charge state

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3.3 Peak List

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Contents

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Chromatogram

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Procedure To insert peaks:

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3.3.3 Saving the Peak List

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Importing and

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3.3.4 Sorting, Filtering

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Peak List

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Software User’s Guide 3-43

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Printing

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Deleting items

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3.4 Deisotoping a Spectrum

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3-46 Applied Biosystems

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Deisotoping a Spectrum

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Software User’s Guide 3-47

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Using the

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Deisotope

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Software User’s Guide 3-49

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3-50 Applied Biosystems

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Returning to the

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3.5 Peak Labeling

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3.5.1 Charge State Labels

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3.5.2 Setting Chromatogram

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Peak Labeling

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Software User’s Guide 3-55

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3-56 Applied Biosystems

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Software User’s Guide 3-57

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3-58 Applied Biosystems

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Labels not

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Charge state

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Software User’s Guide 3-61

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3-62 Applied Biosystems

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Software User’s Guide 3-63

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3-64 Applied Biosystems

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Applying user

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Displaying user

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User labels not

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3.6 Process that Occurs

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During Peak Detection

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Centroiding, and Integration

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3-68 Applied Biosystems

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After peak

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3-70 Applied Biosystems

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Default .SET files

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3-72 Applied Biosystems

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Additional

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Voyager .SET files

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3-74 Applied Biosystems

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Chromatogram Data

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4 Examining

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4.1 Overview

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Types of

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4-4 Applied Biosystems

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4.2 Creating an

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Extracted Ion Chromatogram

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4-6 Applied Biosystems

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Software User’s Guide 4-7

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From the

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Spectrum window

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4.2.2 Creating a Constant

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4-10 Applied Biosystems

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Software User’s Guide 4-11

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4-12 Applied Biosystems

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4.3 Creating an Extracted

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Absorbance Chromatogram

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(XAC) (Mariner Data Only)

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4-14 Applied Biosystems

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4-16 Applied Biosystems

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Noise Filtering/Smoothing

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Software User’s Guide 4-17

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4-18 Applied Biosystems

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Software User’s Guide 4-19

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4.5 Adding and Subtracting

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Software User’s Guide 4-21

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4-22 Applied Biosystems

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(Mariner Data Only)

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Filtering

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Software User’s Guide 4-25

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Evaluating

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Displaying

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4.7 Adjusting the Baseline

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4-28 Applied Biosystems

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Adjusting the Baseline

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Software User’s Guide 4-29

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4.8 Using UV Trace Offset

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Software User’s Guide 4-31

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4-32 Applied Biosystems

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5 Examining

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Spectrum Data

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5.1 Overview

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5-4 Applied Biosystems

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5.3 Manual Calibration

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5-6 Applied Biosystems

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5.3.2 Manually Calibrating

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Manually

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Manual Calibration

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Software User’s Guide 5-9

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Manually matching

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Software User’s Guide 5-11

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Eliminating data

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Software User’s Guide 5-13

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Automatically

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Applying new

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5-16 Applied Biosystems

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Software User’s Guide 5-17

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5-18 Applied Biosystems

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Software User’s Guide 5-19

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Modifying a

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Specifying mass

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5-22 Applied Biosystems

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Software User’s Guide 5-23

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Ensuring that

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Importing a

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5.4 Automatic Calibration

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Automatic

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Mariner data

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Importing

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5-30 Applied Biosystems

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Automatic Calibration

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Software User’s Guide 5-31

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5-32 Applied Biosystems

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Saving the .SET

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Software User’s Guide 5-35

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5.5 Centroiding

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5.6 Mass Deconvolution

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5-38 Applied Biosystems

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Software User’s Guide 5-39

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Converting to

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Software User’s Guide 5-41

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5-42 Applied Biosystems

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Software User’s Guide 5-43

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5-44 Applied Biosystems

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5.8 Adjusting the Baseline

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5-46 Applied Biosystems

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Software User’s Guide 5-47

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5-48 Applied Biosystems

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Correcting the

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(continued)

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Software User’s Guide 5-53

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5-54 Applied Biosystems

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5.9 Truncating a Spectrum

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Truncating a Spectrum

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Software User’s Guide 5-57

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5-58 Applied Biosystems

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Spectrum (Mariner Data Only)

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5-60 Applied Biosystems

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Software User’s Guide 5-61

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Effect on Mariner

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.RST files

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Processed Spectra from the

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Same or Different Data Files

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Software User’s Guide 5-65

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5-66 Applied Biosystems

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6 Using Tools and

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Applications

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6.1 Using the Elemental

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Composition Calculator

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Software User’s Guide 6-3

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6-4 Applied Biosystems

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Electron state for

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If no results are

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6.1.2 Setting Limits

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6-8 Applied Biosystems

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Adding new

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Figure 6-6 Periodic Table

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Software User’s Guide 6-11

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Setting limits for

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Using the Isotope

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Calculator

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6-14 Applied Biosystems

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Using the Isotope Calculator

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Software User’s Guide 6-15

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Add and subtract

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Software User’s Guide 6-17

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If results are not

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6.3 Using the

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Mass Resolution Calculator

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Software User’s Guide 6-21

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6-22 Applied Biosystems

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6-24 Applied Biosystems

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6.5 Using the

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Ion Fragmentation Calculator

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6-26 Applied Biosystems

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Software User’s Guide 6-27

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Calculating the

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Software User’s Guide 6-29

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Creating a

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6.6 Using the Elemental

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Targeting Application

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6-32 Applied Biosystems

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Displaying the

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6.7 Using the Macro Recorder

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6-36 Applied Biosystems

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6.7.2 Recording a Macro

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Assigning a

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6.7.4 Running a Macro

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If the macro

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6.7.5 Deleting a Macro

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6.7.6 Advanced Macro Editing

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Importing when

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Exporting To export macros:

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Using the Macro Recorder

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Software User’s Guide 6-45

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6-46 Applied Biosystems

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7 Data Explorer

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Examples

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7.1 Mariner Data Examples

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Mariner Data Examples

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Software User’s Guide 7-3

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7.1.2 Deconvoluting and

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Evaluating Unresolved

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Chromatographic Peaks

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Creating

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Software User’s Guide 7-7

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7.1.3 Determining if a Peak

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Software User’s Guide 7-9

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7.2 Voyager Data Examples

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Adjusting peak

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Voyager Data Examples

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Software User’s Guide 7-13

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Optimize Mass Accuracy

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Software User’s Guide 7-15

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7-16 Applied Biosystems

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Software User’s Guide 7-17

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7.2.3 Detecting Peaks

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Setting detection

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Basic Settings Tab

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Software User’s Guide 7-21

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Increasing

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8 Viewing

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Voyager PSD Data

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8.1 Displaying PSD Data

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Displaying PSD Data

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Software User’s Guide 8-3

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8-4 Applied Biosystems

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Redisplaying the

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8-6 Applied Biosystems

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Region of

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8.2 Applying Fragment Labels

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Applying Fragment Labels

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Software User’s Guide 8-9

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When to use this

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Overview of

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.CAL file

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Calibrating a PSD Spectrum

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Software User’s Guide 8-11

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8.3.2 Calibrating

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Software User’s Guide 8-13

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8-14 Applied Biosystems

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Matching peaks

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Selecting peaks

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Software User’s Guide 8-17

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Solving and

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Selecting

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Creating PSD

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.CAL files

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8.3.4 Creating PSD

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8-22 Applied Biosystems

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When to change

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8-24 Applied Biosystems

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9 Troubleshooting

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9.1 Overview

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9.2 General Troubleshooting

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Chapter 9 Troubleshooting

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9-4 Applied Biosystems

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General Troubleshooting

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Software User’s Guide 9-5

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9.3 Processing, Tools, and

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Applications Troubleshooting

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Software User’s Guide 9-7

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9-8 Applied Biosystems

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Software User’s Guide 9-9

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9-10 Applied Biosystems

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Calibration Troubleshooting

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Software User’s Guide 9-11

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9-12 Applied Biosystems

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Software User’s Guide 9-13

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9.5 Printing Troubleshooting

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9.6 Peak Detection and

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Labeling Troubleshooting

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9-16 Applied Biosystems

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Software User’s Guide 9-17

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9-18 Applied Biosystems

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Software User’s Guide 9-19

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9-20 Applied Biosystems

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Software User’s Guide 9-21

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9-22 Applied Biosystems

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Appendix

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Warranty period

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Warranty

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Warranties

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Appendix A Warranty

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A-4 Applied Biosystems

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B.1 Isotopes

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Isotopic pattern

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Isotope-limited

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Isotopes

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Software User’s Guide B-5

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B.2 Monoisotopic

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Figure B-7 Average Mass

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Average mass

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B-8 Applied Biosystems

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C.1 Overview

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C.2 Preparing Data

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Before Accessing Macros

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C.3 Accessing the Macros

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C.4 Using the

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Ladder Sequencing Toolbox

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C-6 Applied Biosystems

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Software User’s Guide C-7

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C.5 Using the Peptide

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Fragmentation Toolbox

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Pairs To list ion pairs:

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Software User’s Guide C-11

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C-12 Applied Biosystems

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Software User’s Guide C-13

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C.6 Using the

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Polymer Analysis Toolbox

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C-16 Applied Biosystems

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Calculations

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Preparing data

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MS Fit/MS tag

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Using MS Fit/MS Tag Toolbox

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Software User’s Guide C-19

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C-20 Applied Biosystems

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Numerics and Symbols

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Index-2 Applied Biosystems

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Index-4 Applied Biosystems

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Index-6 Applied Biosystems

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Index-8 Applied Biosystems

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Index-10 Applied Biosystems

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Index-12 Applied Biosystems

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Index-14 Applied Biosystems

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Index-16 Applied Biosystems

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Index-18 Applied Biosystems

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Index-20 Applied Biosystems

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Index-22 Applied Biosystems

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Index-24 Applied Biosystems

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Index-26 Applied Biosystems

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Index-28 Applied Biosystems

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Index-30 Applied Biosystems

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