HP Data Explorer 4 Series Uživatelský manuál stáhnout pdf (Strana 148)

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Chapter 3 Peak Detection and Labeling

3-50 Applied Biosystems

Example Figure 3-19 and Figure 3-20 illustrate the effects of

deisotoping.

Before deisotoping (Figure 3-19), the spectrum includes an

isotope pattern with four detected peaks.

Figure 3-19 Spectrum Before Deisotoping

After deisotoping (Figure 3-20), the trace includes two

labeled centroid bars that represent monoisotopic masses,

indicating that the original trace represents two isotopic

envelopes. The intensity of a centroid bar is proportional to

the area of all peaks in the isotope cluster.

Figure 3-20 Spectrum After Deisotoping

1 2 ... 143 144 145 146 147 148 149 150 151 152 153 ... 446 447

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