HP Data Explorer 4 Series Uživatelský manuál stáhnout pdf (Strana 210)

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101

Chapter 5 Examining Spectrum Data

5-6 Applied Biosystems

The manual calibration feature provides two modes for peak

matching:

• Automatic—The software automatically compares

reference masses to observed masses, and lists peaks

that are within the specified peak matching criteria.

• Manual—You manually select an observed mass, then

select the reference mass for comparison.

Hint: It is often useful to automatically perform a match and

fit first, then manually adjust the fit as needed.

Calibration

references files

(.REF) provided

Default calibration reference files are provided with the

software. You can use these files as a starting point and add

delete reference masses as needed.

For information, see Section 5.3.3, Creating or Modifying a

Calibration Reference File (.REF).

When to use

manual

calibration

Use manual calibration when you:

• Calibrate Voyager data

• Calibrate Mariner data and any of the following occur:

• You have one or only a few spectra to calibrate

• You do not know in advance which reference

masses to use

• You do not know in advance if the quality of the

reference mass signals is acceptable

• You want to fine-tune the results of an automatic

calibration

For information on automatic calibration, see Section 5.4,

Automatic Calibration.

Accurate mass

measurements

Before performing an internal calibration, refer to the:

• Mariner Workstation User’s Guide

• Voyager Biospectrometry Workstation User’s Guide

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